[2020] MALDI-MS analysis of disaccharide isomers using graphene oxide as MALDI matrix
Author
관리자
Date
2020-10-01 07:23
Views
334
링크 : https://doi.org/10.1016/j.foodchem.2020.128356
일자 : 2020.10
제목 : MALDI-MS analysis of disaccharide isomers using graphene oxide as MALDI matrix
Dabin Lee1, Yeoseon Kim1, Iqbal Jalaludin1, Huu-Quang Nguyen1, Minsun Kim2, Jungju Seo2, Kyoung-Soon Jang3, and Jeongkwon Kim1,4*
1Department of Chemistry, Chungnam National University, Daejeon, Republic of Korea
2Center for Scientific Instrumentation, Korea Basic Science Institute, Daejeon, Republic of Korea
3Biomedical Omics Center, Korea Basic Science Institute, Cheongju, Republic of Korea
4Graduate School of New Drug Discovery and Development, Chungnam National University,
Daejeon, Republic of Korea
Abstract
Disaccharides are sugars composed of two monosaccharides joined by a glycosidic linkage. The specific properties of a disaccharide depend on the type of the glycosidic linkage and the identity of the two component monosaccharides. In this work, seven disaccharide isomers (gentiobiose, isomaltose, melibiose, lactose, maltose, cellobiose, and sucrose) were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using a graphene oxide matrix. Each disaccharide was identified by its unique cleavage pattern. To determine the feasibility of quantitative analyses based on specific fragment patterns, mixtures of sucrose with cellobiose or maltose were prepared at different ratios and analyzed by MALDI-MS, where a strong linear correlation was observed between the relative peak intensity of the sucrose fragment peak at m/z 185 and the amount of sucrose in the mixture. The calibration curve was successfully applied to obtain the relative amount of maltose and sucrose in four different honey samples.
일자 : 2020.10
제목 : MALDI-MS analysis of disaccharide isomers using graphene oxide as MALDI matrix
Dabin Lee1, Yeoseon Kim1, Iqbal Jalaludin1, Huu-Quang Nguyen1, Minsun Kim2, Jungju Seo2, Kyoung-Soon Jang3, and Jeongkwon Kim1,4*
1Department of Chemistry, Chungnam National University, Daejeon, Republic of Korea
2Center for Scientific Instrumentation, Korea Basic Science Institute, Daejeon, Republic of Korea
3Biomedical Omics Center, Korea Basic Science Institute, Cheongju, Republic of Korea
4Graduate School of New Drug Discovery and Development, Chungnam National University,
Daejeon, Republic of Korea
Abstract
Disaccharides are sugars composed of two monosaccharides joined by a glycosidic linkage. The specific properties of a disaccharide depend on the type of the glycosidic linkage and the identity of the two component monosaccharides. In this work, seven disaccharide isomers (gentiobiose, isomaltose, melibiose, lactose, maltose, cellobiose, and sucrose) were analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using a graphene oxide matrix. Each disaccharide was identified by its unique cleavage pattern. To determine the feasibility of quantitative analyses based on specific fragment patterns, mixtures of sucrose with cellobiose or maltose were prepared at different ratios and analyzed by MALDI-MS, where a strong linear correlation was observed between the relative peak intensity of the sucrose fragment peak at m/z 185 and the amount of sucrose in the mixture. The calibration curve was successfully applied to obtain the relative amount of maltose and sucrose in four different honey samples.